5(6)-FAM

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Synonyms: 5 - (and - 6) - Carboxyfluorescein

Functional Activity:

Mixed isomer of 5 and 6-FAM. Fluorescein is one of the most popular green fluorescent reagents used for in situ labeling of peptides, proteins and nucleotides. It reacts with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein has relatively high absorptivity, excellent fluorescence quantum yield, good water solubility, and an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. The cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume. It has also been used to prepare various small fluorescent molecules.

Technical Data:

M.Wt: 376.32

Formula: C₂₁H₁₂ O₇

Excitation (nm): 494

Emission (nm): 519

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at room temperature and protect from light.

CAS No.: 72088-94-9

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References for 5(6)-FAM :

1. Watson D, Zhang GY, Sartor M, Alexander SI. (2004) "pruning" of alloreactive cd4+ t cells using 5- (and 6-)carboxyfluorescein diacetate succinimidyl ester prolongs skin allograft survival. J Immunol, 173, 6574.

2. Murthy SN, Zhao YL, Sen A, Hui SW. (2004) Cyclodextrin enhanced transdermal delivery of piroxicam and carboxyfluorescein by electroporation. J Control Release, 99, 393.