Disease:

Mouse myeloma, hybridoma

Origin:

a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8

Species:

Mus musculus BALB/C

Tissue:

Spleen

Morphology:

Lymphoblast

Properties:

Suspension

Cytogenic data:

Patient:

Medium:

DMEM + 10% FBS

Subculture:

1:3 to 1:5, 5% CO2; 37�C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Frozen vial

USD206.0

1. Martinelle, K. and Haggstrom, L. Effects of NH4+ and K+ on the energy metabolism in Sp2/0-Ag14 myeloma cells. Cytotechnology, 29: 45-53, 1999.

2. Guerin, P. J., Furtak, T., Eng, K., and Gauthier, E. R. Oxidative stress is not required for the induction of apoptosis upon glutamine starvation of Sp2/0-Ag14 hybridoma cells. Eur J Cell Biol, 85: 355-365, 2006.

3. Zhu, M. S., Liu, D. G., Cheng, H. Q., Xu, X. Y., and Li, Z. P. Expression of exogenous NF-IL6 induces apoptosis in Sp2/0-Ag14 myeloma cells. DNA Cell Biol, 16: 127-135, 1997.

4. Kang, J. S., Choi, C. M., and Yun, I. Effects of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured mouse myeloma cell line Sp2/0-Ag14. Biochim Biophys Acta, 1281: 157-163, 1996.

5. Liu, Z. Q., Wu, A. F., and Wu, T. T. Short gene inversion involving two adjacent heavy chain joining minigenes and one heavy chain diversity minigene in the nonsecretor Sp2/0-Ag14 myeloma cell line. Nucleic Acids Res, 15: 4688, 1987.

6. Sorokina, E. A., Kozhukharova, I. V., and Grinchuk, T. M. [Karyotypic characteristics of the murine myeloma line sp2/0-Ag14]. Tsitologiia, 28: 623-628, 1986.

7. Lacy, M. J. and Voss, E. W., Jr. A modified method to induce immune polyclonal ascites fluid in BALB/c mice using Sp2/0-Ag14 cells. J Immunol Methods, 87: 169-177, 1986.