Disease:

Fibroblast spontaneously transformed

Origin:

UMNSAH/DF-1 is a spontaneously immortalized chicken cell line derived from 10 day old East Lansing Line (ELL-0) eggs.

Species:

Gallus gallus, chicken

Tissue:

Embryo

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%

Strain:

East Lansing Line (ELL-0), 10 days gestation

Medium:

AddexBio-Formulated DMEM + 10% Fetal Bovine Serum (FBS).

Subculture:

1:2 to 1:10 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 39 °C (Max. 40°C, Min. 38°C)

Freezing Medium:

Complete culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility: