DF-1 cells
Catalog #:
P0011015
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Frozen vial | USD355.0 |
Disease:
Fibroblast spontaneously transformed
UMNSAH/DF-1 is a spontaneously immortalized chicken cell line derived from 10 day old East Lansing Line (ELL-0) eggs.
Gallus gallus, chicken
Embryo
Fibroblast
Adherent
Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
East Lansing Line (ELL-0), 10 days gestation
AddexBio-Formulated DMEM + 10% Fetal Bovine Serum (FBS).
1:2 to 1:10 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 39 °C (Max. 40°C, Min. 38°C)
Complete culture medium supplemented with 7.5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
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Applications:
The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.