5-FTSC

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Synonyms: Fluorescein - 5 - thiosemicarbazide

Functional Activity:

5-FTSC can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). 5-FTSC has been used to make a wide variety of biomolecules such as L-aspartase-a-decarboxylase, enzyme. It has also been used for labeling of cell-surface functional groups such as glycophorins.

Technical Data:

M.Wt: 421.4

Formula: C₂₁H₁₅N₃O₅S

Excitation (nm): 492

Emission (nm): 516

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at room temperature and protect from light.

CAS No.: 76863-28-0

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References for 5-FTSC :

1. Ahn B, et al. (1987). Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels. Anal Biochem 161, 245-57;

2. Atha DH, et al. (1984). Interaction and thermal stability of fluorescent labeled derivatives of thrombin and antithrombin III. Biochim Biophys Acta 785, 1-6.