5-TMRIA

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Synonyms: Tetramethylrhodamine - 5 - iodoacetamide

Functional Activity:

Tetramethylrhodamine iodoacetamides (TMRIA) are thiol-selective reactive dyes that are used to label proteins via the cysteine residues. 5-TMRIA, the pure 5-isomer of TMRIA, is increasingly preferred for some particular applications since the mixed isomers of TMRIA may give different results from batch to batch due to the varying ratios and different reactivities of the two isomers.

Technical Data:

M.Wt: 569.39

Formula: C₂₆H₂₆I₃N₃O₄

Excitation (nm): 541

Emission (nm): 567

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

CAS No.: 114458-99-0

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References for 5-TMRIA :

1. Martyn DA, et al. (2001). Ca2+- and cross-bridge-dependent changes in N- and C-terminal structure of troponin C in rat cardiac muscle. Biophys J 80, 360-70;

2. Ajtai K and Burghardt TP (1995). Conformation of xanthene dyes in the sulfhydryl 1 binding site of myosin. 2. Biochemistry 34, 15943-52.