5(6)-FAM azide

Catalog #:

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Synonyms: 5- (and -6) ? carboxyfluorescein azide

Functional Activity:

5(6)-FAM azide is an excellent alkyne-reactive fluorescent reagent for labeling azide-containing biological molecules through click chemitry. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur ? the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls ? functional groups that are not unique ? click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.

Technical Data:

M.Wt: 458.43

Formula: C₂₄H₁₈N₄O₆

Excitation (nm): 495

Emission (nm): 519

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

CAS No.: N/A

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References for 5(6)-FAM azide :

1. Angew. Chem. Int. Ed. 2008, 47, 3442 ?3444; Angew. Chem. Int. Ed. 2008, 47, 8350-8358; Tetrahedron Lett. 2005, 46, 1691-1695.

Certificate of Analysis/MSDS is available upon request