5-IAF
Catalog #:
ABBFO1059Shipping advice:
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ABBFO1059-25mg | USD129.0 |
Synonyms: 5 - Iodoacetamidofluorescein

Functional Activity:
Like maleimides, iodoacetamides are also among the most frequently used reagents for thiol modification. 5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein?5-maleimide.
Technical Data:
M.Wt: 515.25
Formula: C22H14INO6
Excitation (nm): 492
Emission (nm): 515
Solubility: Soluble in DMSO, DMF
Purity: >95% (HPLC)
Shipping Conditions: Room temperature
Storage: Store and desiccate at -20°C and protect from light.
CAS No.: 63368-54-7
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References for 5-IAF :
1. Muddukrishna SN, Chen A, Qi P, Smolenski MA. (1995) Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique. Appl Radiat Isot, 46, 1015.
2. Lopina OD, Pindel EV, Boldyrev AA. (1990) Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides. FEBS Lett, 266, 75.