5-IAF

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Synonyms: 5 - Iodoacetamidofluorescein

Functional Activity:

Like maleimides, iodoacetamides are also among the most frequently used reagents for thiol modification. 5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein?5-maleimide.

Technical Data:

M.Wt: 515.25

Formula: C₂₂H₁₄INO₆

Excitation (nm): 492

Emission (nm): 515

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

CAS No.: 63368-54-7

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References for 5-IAF :

1. Muddukrishna SN, Chen A, Qi P, Smolenski MA. (1995) Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique. Appl Radiat Isot, 46, 1015.

2. Lopina OD, Pindel EV, Boldyrev AA. (1990) Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides. FEBS Lett, 266, 75.