5 - ROX

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Synonyms: 5 - Carboxy - X - rhodamine

Functional Activity:

5-ROX is the purified single isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 5-ROX is often used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine? rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red® conjugates.

Technical Data:

M.Wt: 635.8

Formula: C₃₉H₄₅ N₃ O₅

Excitation (nm): 567

Emission (nm): 591

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at -20°C and protect from light.

CAS No.: N/A

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References for 5 - ROX :

1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.

2. Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C. (2004) Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence. Langmuir, 20, 6834.