5 - ROX
Catalog #:
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Synonyms: 5 - Carboxy - X - rhodamine

Functional Activity:
5-ROX is the purified single isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 5-ROX is often used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine? rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas RedŽ conjugates.
Technical Data:
M.Wt: 635.8
Formula: C39H45 N3 O5
Excitation (nm): 567
Emission (nm): 591
Solubility: Soluble in DMSO, DMF
Purity: >95% (HPLC)
Shipping Conditions: Room temperature
Storage: Store at -20°C and protect from light.
CAS No.: N/A
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References for 5 - ROX :
1. Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. (2005) Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci U S A, 102, 5926.
2. Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C. (2004) Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence. Langmuir, 20, 6834.