5-FAM

Catalog #:

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Synonyms: 5 - Carboxyfluorescein

Functional Activity:

5-FAM is the purified single isomer of carboxyfluorescein. . It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.

Technical Data:

M.Wt: 376.32

Formula: C₂₁H₁₂ O₇

Excitation (nm): 494

Emission (nm): 518

Solubility: Soluble in DMSO, DMF

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store at room temperature and protect from light.

CAS No.: 76823-03-5

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References for 5-FAM :

1. Morono Y, Takano S, Miyanaga K, Tanji Y, Unno H, Hori K. (2004) Application of glutaraldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate. Biotechnol Lett, 26, 379.

2. David R, Machova Z, Beck-Sickinger AG. (2003) Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8. Biol Chem, 384, 1619.