EGTA
Catalog #:
ION0006| IN0006-1g | USD25.0 |
Synonyms: EGTA, ethylene glycol tetraacetic acid
IUPAC Name:
ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
Functional Activity:
EGTA (ethylene glycol tetraacetic acid) is a polyamino carboxylic acid, a chelating agent that is related to the better known EDTA, but with a much higher affinity for calcium than for magnesium ions. It is useful for making buffer solutions that resemble the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium.
The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91. See Qin et al. for an example of a pKa calculation.
EGTA has also been used experimentally for the treatment of animals with cerium poisoning and for the separation of thorium from the mineral monazite. EGTA is used as a compound in elution buffer in the protein purification technique known as Tandem Affinity Purification (TAP), in which recombinant fusion proteins are bound to calmodulin beads and eluted out by adding EGTA.
EGTA is often employed in dentistry and endodontics for the removal of smear layer.
Technical Data:
M.Wt: 380.35
Formula: C14H24 N2O10
Solubility: Soluble to 100 mM in 1M NaOH
Purity: >99%
Storage: Dessicate at RT
CAS No.: 67-42-5
Related Products by Target:
References for EGTA:
1. Hastings, A. D., Herbert, S. P., Gawler, D., and Walker, J. H. Association with actin mediates the EGTA-resistant binding of cytosolic phospholipase A2-alpha to the plasma membrane of activated platelets. Cell Biol Int, 33: 83-91, 2009.
2. Tei, L., Botta, M., Lovazzano, C., Barge, A., Milone, L., and Aime, S. 1H and 17O NMR relaxometric study in aqueous solution of Gd(III) complexes of EGTA-like derivatives bearing methylenephosphonic groups. Magn Reson Chem, 46 Suppl 1: S86-93, 2008.
3. Tei, L., Baranyai, Z., Botta, M., Piscopo, L., Aime, S., and Giovenzana, G. B. Synthesis and solution thermodynamic study of rigidified and functionalised EGTA derivatives. Org Biomol Chem, 6: 2361-2368, 2008.
4. Zhao, Q., Yuan, S., Wang, X., Zhang, Y., Zhu, H., and Lu, C. Restoration of mature etiolated cucumber hypocotyl cell wall susceptibility to expansin by pretreatment with fungal pectinases and EGTA in vitro. Plant Physiol, 147: 1874-1885, 2008.
5. Samai, M., Hague, T., Naughton, D. P., Gard, P. R., and Chatterjee, P. K. Reduction of paraquat-induced renal cytotoxicity by manganese and copper complexes of EGTA and EHPG. Free Radic Biol Med, 44: 711-721, 2008.
6. Boaz, S. M., Dominguez, K., Shaman, J. A., and Ward, W. S. Mouse spermatozoa contain a nuclease that is activated by pretreatment with EGTA and subsequent calcium incubation. J Cell Biochem, 103: 1636-1645, 2008.
