Sodium-Calcium Exchanger (NCX) Monoclonal Antibody

Catalog #:

Ab001

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4.0 mg/mL

USD309.0

Product Description:

The monoclonal antibody against the canine Na+-Ca2+-exchanger, recognizes two neighboring but non-overlapping sequences of the hydrophilic region of the exchanger, connecting the putative transmembrane segments 5 and 6. The antibody was evaluated for specificity and potency by immunoblotting, and immunoprecipitation (see reference). It recognizes virtually only NCX1 in monkey, horse, dog, rat, bovine and mouse tissue. At higher concentrations it reacts faintly with NCX2, whereas NCX3 is not recognized. Both splice variants with exon A (heart, nervous system) and exon B (kidney, glia) are recognized.

Protein Name:

Sodium-Calcium Exchanger (NCX)

Product:

Monoclonal anti-Na+-Ca2+-exchanger (NCX)

Form:

In sterile solution.

IgG Subtype:

IgG1

Quantity:

4.0 mg/mL

Immunoblot:

1:5000 (use milk for blocking). A strong 70 kDa band is found in addition to the 116 kDa of the integral exchanger. Often the HMW band is a double band 116/112 kDa band.)

Immunoprecipitation:

1:500

Immunohistochemistry:

1:2500 The titer was determined using the indirect-peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.

Storage:

After reconstitution freeze in small aliquots (e.g. 5 uL) and keep at -80 degree C (or at least -20 degree C). For continuous use keep at 4 degree C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

Related Products by Target:

KB-R7943 mesylate

References for NCX1 Antibody:

1. Dong, H., Sellers, Z. M., Smith, A., Chow, J. Y., and Barrett, K. E. Na(+)/Ca(2+) exchange regulates Ca(2+)-dependent duodenal mucosal ion transport and HCO(3)(-) secretion in mice. Am J Physiol Gastrointest Liver Physiol, 288: G457-465, 2005.

2. Dong, H., Shim, K. N., Li, J. M., Estrema, C., Ornelas, T. A., Nguyen, F., Liu, S., Ramamoorthy, S. L., Ho, S., Carethers, J. M., and Chow, J. Y. Molecular mechanisms underlying Ca2+-mediated motility of human pancreatic duct cells. Am J Physiol Cell Physiol, 299: C1493-1503.

3. Gomez-Villafuertes, R., Mellstrom, B., and Naranjo, J. R. Searching for a role of NCX/NCKX exchangers in neurodegeneration. Mol Neurobiol, 35: 195-202, 2007.

4. Jeffs, G. J., Meloni, B. P., Bakker, A. J., and Knuckey, N. W. The role of the Na(+)/Ca(2+) exchanger (NCX) in neurons following ischaemia. J Clin Neurosci, 14: 507-514, 2007.

5. Moon, H. S., Choi, E., and Hyun, C. The cardiac sodium-calcium exchanger gene (NCX-1) is a potential canine cardiac biomarker of chronic mitral valvular insufficiency. J Vet Intern Med, 22: 1360-1365, 2008.

6. Nam, S. J., Han, S. H., Kim, H. W., and Hyun, C. The cardiac biomarker sodium-calcium exchanger (NCX-1) can differentiate between heart failure and renal failure: a comparative study of NCX-1 expression in dogs with chronic mitral valvular insufficiency and azotemia. J Vet Intern Med, 24: 1383-1387.

7. Poburko, D., Fameli, N., Kuo, K. H., and van Breemen, C. Ca2+ signaling in smooth muscle: TRPC6, NCX and LNats in nanodomains. Channels (Austin), 2: 10-12, 2008.

8. Porzig, H., Li, Z., Nicoll, D. A., and Philipson, K. D. Mapping of the cardiac sodium-calcium exchanger with monoclonal antibodies. Am J Physiol, 265: C748-756, 1993.

9. Reppel, M., Fleischmann, B. K., Reuter, H., Sasse, P., Schunkert, H., and Hescheler, J. Regulation of the Na+/Ca2+ exchanger (NCX) in the murine embryonic heart. Cardiovasc Res, 75: 99-108, 2007.