IEC-6 cells
Catalog #:
P0021001Shipping advice:
Use only Express Delivery (overnight) for cell lines. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
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Origin:
Normal small intestineSpecies:
Rattus norvegicus (rat)Tissue:
Small intestineMorphology:
EpithelialProperties:
AdherentCytogenic data:
NormalMedium:
DMEM + 10% FBSSubculture:
1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree CFreezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level:
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: NegativeSterility:
Pathogens:
References for IEC-6 cells:
1. Fujii, K., Luo, Y., Sasahira, T., Denda, A., Ohmori, H., and Kuniyasu, H. Co-treatment with deoxycholic acid and azoxymethane accelerates secretion of HMGB1 in IEC6 intestinal epithelial cells. Cell Prolif, 42: 701-709, 2009.
2. Mayer, K., Vreemann, A., Qu, H., and Brix, K. Release of endo-lysosomal cathepsins B, D, and L from IEC6 cells in a cell culture model mimicking intestinal manipulation. Biol Chem, 390: 471-480, 2009.
3. Sasaki, T., Shimura, H., Sasahira, T., Fujii, K., and Kuniyasu, H. High concentration of deoxycholic acid abrogates in vitro transformation of IEC6 intestinal cells by azoxymethane. J Exp Clin Cancer Res, 24: 625-631, 2005.
4. Sasaki, T., Yoshida, K., Shimura, H., Ichiba, M., Sasahira, T., Shimomoto, T., Denda, A., and Kuniyasu, H. Inhibitory effect of linoleic acid on transformation of IEC6 intestinal cells by in vitro azoxymethane treatment. Int J Cancer, 118: 593-599, 2006.
5. Ouko, L., Ziegler, T. R., Gu, L. H., Eisenberg, L. M., and Yang, V. W. Wnt11 signaling promotes proliferation, transformation, and migration of IEC6 intestinal epithelial cells. J Biol Chem, 279: 26707-26715, 2004.
6. Uzzau, S., Lu, R., Wang, W., Fiore, C., and Fasano, A. Purification and preliminary characterization of the zonula occludens toxin receptor from human (CaCo2) and murine (IEC6) intestinal cell lines. FEMS Microbiol Lett, 194: 1-5, 2001.
7. Fukushima, K., Sasaki, I., Sato, S., Sasano, H., Krozowski, Z., and Matsuno, S. Induction of mineralocorticoid receptor by sodium butyrate in small intestinal (IEC6) and colonic (T84) epithelial cell lines. Dig Dis Sci, 44: 1571-1578, 1999.
8. Fukushima, K., Sasaki, I., Hasegawa, H., Takahashi, K., Naito, H., Funayama, Y., and Matsuno, S. Sodium butyrate-induced liver-type alkaline phosphatase activity in a small intestinal epithelial cell line, IEC6. Dig Dis Sci, 43: 1116-1123, 1998.
