STO cells
Catalog #:
P0011006
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| Frozen vial | USD450.0 |
Disease:
Normal
Origin:
The STO cell line was derived from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. Used routinely as feeder layers prepared by irradiation or mitomycinc treatment for maintenance of teratocarcinoma stem cells in the undifferentiated state. The cells have been tested and found negative for ectromelia virus (mousepox).
Species:
Mus musculus, mouse
Embryo
Fibroblast
Adherent
Not specified
Embryo, SIM (Sandos Inbred Mice)
AddexBio-formulated DMEM (C0003-01)
+ 10% FBS
Split sub-confluent cultures (70-80%) at a seed ratio of 1:3 to 1:6, i.e. seeding at 2-4x10,000 cells/cm^2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: STO (AddexBio P0011006)
Tissue:
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Citation Format:
References for STO cells:
1. Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci U S A. 1975;72(4):1441-1445. PMID: 1055416
