PC-12Adh cells
Catalog #:
C0032002
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Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
Disease:
Pheochromocytoma
Origin:
The PC-12 cell line was derived from a transplantable rat pheochromocytoma.
Species:
Rattus norvegicus, rat
Adrenal gland
Polygonal
Adherent, small clusters
Male
F-12K + 2.5% FBS + 15% Horse Serum.
Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Tissue:
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Citation Format:
References for PC-12Adh cells:
1. Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-2428. PMID: 1065897
