DLD-1 cells
Catalog #:
C0009007
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| Frozen vial | USD420.0 |
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Disease:
Colorectal adenocarcinoma; Dukes' type C
Carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; colon antigen 3; keratin
Homo sapiens
Colon
Epithelial
Adherent
This is a pseudodiploid human cell line
Male, adult
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
1:3 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: DLD-1 (AddexBio C0009007)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
Protein Expression:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
Isoenzymes:
ES-D, 1-2;
G6PD, B;
PEP-D, 1;
PGD, A;
PGM1, 1;
PGM3, 1
DNA Profile (STR):
Amelogenin: X,Y;
CSF1PO: 11,12;
D13S317: 8,11;
D16S539: 12,13;
D5S818: 13;
D7S820: 10,12;
THO1: 7,9.3;
TPOX: 8,11;
vWA: 18,19
Citation Format:
Related cells by Organ:
References for DLD-1 cells:
1. Bachmann, M., Paulukat, J., Pfeilschifter, J., and Muhl, H. Molecular mechanisms of IL-18BP regulation in DLD-1 cells: pivotal direct action of the STAT1/GAS axis on the promoter level. J Cell Mol Med, 13: 1987-1994, 2009.
2. Orlando, A., Messa, C., Linsalata, M., Cavallini, A., and Russo, F. Effects of Lactobacillus rhamnosus GG on proliferation and polyamine metabolism in HGC-27 human gastric and DLD-1 colonic cancer cell lines. Immunopharmacol Immunotoxicol, 31: 108-116, 2009.
3. Raju, J., Bielecki, A., Caldwell, D., Lok, E., Taylor, M., Kapal, K., Curran, I., Cooke, G. M., Bird, R. P., and Mehta, R. Soy isoflavones modulate azoxymethane-induced rat colon carcinogenesis exposed pre- and postnatally and inhibit growth of DLD-1 human colon adenocarcinoma cells by increasing the expression of estrogen receptor-beta. J Nutr, 139: 474-481, 2009.
4. Shikano, N., Ogura, M., Okudaira, H., Nakajima, S., Kotani, T., Kobayashi, M., Nakazawa, S., Baba, T., Yamaguchi, N., Kubota, N., Iwamura, Y., and Kawai, K. Uptake of 3-[125I]iodo-alpha-methyl-L-tyrosine into colon cancer DLD-1 cells: characterization and inhibitory effect of natural amino acids and amino acid-like drugs. Nucl Med Biol, 37: 197-204.
5. Uzun, Y., Akdogan, E., Ozdemir, F., and Ovali, E. The effects of heparin on DLD-1 colon cancer cell line. Bratisl Lek Listy, 110: 3-6, 2009.
6. Yang, B., Chuang, H., and Yang, K. D. Sialylated glycans as receptor and inhibitor of enterovirus 71 infection to DLD-1 intestinal cells. Virol J, 6: 141, 2009.
